Since the autopsy i have been considering different possibilities as to why my beans germination was unsuccessful. Initially i had thought that glucose would help the bean by giving it more energy, although i am aware that the bean has all the energy it needs for germination ready inside. The mixture was quite strong, about 1:10 glucose / water, a combination which works for me when i need high levels of energy at times of physical exertion. I had presumed that the bean would also be involved in a high level of physical exertion in germination, and the glucose would help!
I considered possibilitythat perhaps the glucose had made it too sticky for the bean to emerge, although the autopsy revealed that the bean had made no progress whatsoever on the germination front. Although there was evidence of moisture in the bean, perhaps there wasn't enough at its critical stages of germination.
A feasible theory on it's failure to germinate could be related to osmosis which would affect the water balance of the bean and absorption of moisture. If the sugar content of the water outside the bean was greater than the bean itself then water could potentially be drawn out of the bean.
To test whether the water was just too high in glucose i conducted a further experiment. I put a cube of potato in the remaining glucose solution, and one of the same size in water. If the water was easily absorbed the potato will get bigger, if it shrinks the water is evidently being drawn out of the bean. Interestingly over 12 hours, the water potato got bigger, and the glucose potato got very slightly smaller, showing that the glucose solution actually drew moisture out the potato.
Instead of the glucose helping germination, the concentration was so high that it actually hindered the absorption of moisture essential for growth. Although i had intended to help growth, i inadvertently jeopardised any chance of life that my beans may have had!
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